Revision 3

#7988

Store at -20C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Description

Jurkat cells are treated with 100 nM calyculin A and 1 mM pervanadate to inhibit multiple serine/threonine and tyrosine phosphatases, respectively and upregulate protein phosphorylation. Treated Jurkat cells were lysed in 1X cell lysis buffer and lysates were lyophilized.

Storage

Supplied as lyophilized product. Keep at -20°C for short term storage or at -80°C for longer periods. Avoid repeated freeze-thaw cycles.

Background

PathScan® Sandwich ELISA is a convenient tool for the analysis of protein expression as well as post-translational modifications (such as phosphorylation and acetylation) allowing the researcher to study signal transduction. PathScan® Sandwich ELISA Control Phospho Cell Extracts I can be used not only to troubleshoot the experiment but also provides the means to standardize the signal allowing the comparison of results obtained from different plates or experiments.

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PathScan is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

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Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

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ELISA analysis of PathScan® Sandwich ELISA Control Phospho Cell Extracts I using multiple PathScan® Sandwich ELISA kits. Samples were prepared using the standard ELISA protocol (attached) to a final concentration of 0.25 mg/ml and assayed using the indicated ELISA kits.
undefined Image 1: PathScan<sup>®</sup> Sandwich ELISA Control Phospho Cell Extract I
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.