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PathScan^® Total EpCAM Sandwich ELISA Kit

PathScan^® Total EpCAM Sandwich ELISA Kit #85626

Filter:

ELISA

Supporting Data

REACTIVITY

Application Key:

ELISA-ELISA

Species Cross-Reactivity Key:

H-Human

Product Information

Product Description

The PathScan^® Total EpCAM Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of EpCAM protein. An EpCAM rabbit mAb has been coated onto the microwells. After incubation with cell lysates, the EpCAM proteins are captured by the coated antibody. Following extensive washing, an EpCAM mouse detection mAb is added to detect captured EpCAM proteins. Anti-mouse, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for the developed color is proportional to the quantity of EpCAM protein.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

PathScan^® Total EpCAM Sandwich ELISA Kit detects endogenous levels of EpCAM protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

Background

Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca²⁺-independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). Research studies identified EpCAM as one of the first tumor-associated antigens, and it has long been a marker of epithelial and tumor tissue. Investigators have shown that EpCAM is highly expressed in cancer cells (reviewed in 2,3).

Database Links

UniProt ID: P16422

Entrez-Gene Id: 4072

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PathScan is a registered trademark of Cell Signaling Technology, Inc.

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PathScan® Total EpCAM Sandwich ELISA Kit #85626