Cat. # | Size | Price | Inventory |
---|---|---|---|
7316C | 1 Kit (96 assays) |
When ordering five or more kits, please contact us for processing time and pricing.
Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.
REACTIVITY | H M |
Product Includes | Volume | Solution Color | |||
---|---|---|---|---|---|
Phospho-S6 Ribosomal Protein (S235/236) Rabbit mAb Coated Microwells | 96 tests | ||||
S6 Ribosomal Protein Mouse Detection mAb | 1 ea | Green (Lyophilized) | |||
Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated) | 1 ea | Red (Lyophilized) | |||
Detection Antibody Diluent | 5.5 ml | Green | |||
HRP Diluent | 5.5 ml | Red | |||
Luminol/Enhancer Solution | 3 ml | ||||
Stable Peroxide Buffer | 3 ml | ||||
Sealing Tape | 2 ea | ||||
ELISA Wash Buffer (20X) 9801 | 25 ml | ||||
ELISA Sample Diluent | 25 ml | Blue | |||
Cell Lysis Buffer (10X) 9803 | 15 ml |
Product Information
NOTE: Refer to product-specific datasheets for assay incubation temperature. This chemiluminescent ELISA is offered in low volume microplates. Only 50 µl of samples or reagents are required in each microwell.
NOTE: Prepare solutions with purified water.
*NOTE: Some PathScan® ELISA Kits may include HRP-Linked Streptavidin in place of HRP-Linked Antibody.
posted November 2013
revised July 2024
Protocol Id: 66
One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein (4,5).
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