Revision 1

#35913

Store at -20C

Human CSF-1/M-CSF Recombinant Protein

10 µg

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

MW (kDa):
18.2

UniProt ID:
#P09603

Entrez-Gene Id:
1435

Background

Colony stimulating factor 1 (CSF-1)/macrophage colony stimulating factor (M-CSF) is produced by fibroblasts, endothelial cells, stromal cells, macrophages, osteoblasts, and other cell types (1). CSF-1/M-CSF is required for growth and differentiation of monocytes and macrophages (1,2). CSF-1/M-CSF polarizes macrophages into the M2 phenotype where anti-inflammatory IL-10 is produced, rather than the M1 phenotype where inflammatory cytokines are produced. CSF-1/M-CSF also recruits monocytes and enhances angiogenesis by inducing VEGF production (1,2). CSF-1/M-CSF binds to its receptor CSF1R; downstream signaling involves PI3K/Akt, Erk, and Stats 1, 3, and 5 (1,3). An increase in CSF-1/M-CSF expression may contribute to cancer progression, and high plasma CSF-1/M-CSF levels are associated with rheumatoid arthritis (1,4,5).

Endotoxin

Endotoxin levels are less than or equal to 1 EU / 1 μg hCSF-1/M-CSF.

Purity

A greater than or equal to 95% purity was determined by SDS-PAGE.

Source / Purification

Recombinant human CSF-1/M-CSF was expressed in E. coli and is supplied in a lyophilized form.

Bioactivity

The bioactivity of recombinant hCSF-1/M-CSF was determined in an NFS-60 cell proliferation assay. The ED50 of each lot is less than or equal to 10 ng/mL.

Background References

Cross-Reactivity Key

H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

#35913

Human CSF-1/M-CSF Recombinant Protein

CST Logo
Serial dilutions of Human CSF-1/M-CSF Recombinant Protein were added to NFS-60 cells. Cell proliferation was measured and the linear portion of the curve was used to calculate the ED50.
undefined Image 1: Human CSF-1/M-CSF Recombinant Protein
The purity of Human CSF-1/M-CSF Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant hCSF-1/M-CSF and staining with Coomassie Blue. hCSF-1/M-CSF is a homodimer with a predicted total molecular weight (MW) of 36.4 kDa with each subunit equaling 18.2 kDa.
undefined Image 2: Human CSF-1/M-CSF Recombinant Protein
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.