PIP4K2C (E7P3S) Rabbit mAb (PE Conjugate) #95735
- F
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Description
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu321 of human PIP4K2C protein.
Background
Phosphatidylinositol-5-phosphate 4-kinases (PIP4Ks) synthesize phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2), a key precursor in phosphoinositide signaling that directly modulates the activity of signaling proteins and cellular processes. There are two subfamilies of PIP kinases, type I and II, that generate PtdIns(4,5)P2 from distinct substrate pools. PIP4 type I kinases use PtdIns5P as a substrate, whereas PIP5 type II kinases use PtdIns4P (1,2). In mammalian cells, three isoforms of each PIP4K and PIP5K subfamily, encoded by distinct genes, have been characterized (3-7). All PIP kinases are stimulated by phosphatidic acid, extensively regulated by ARF and Rho GTPases, and inhibited by protein kinase A and PI-stimulated autophosphorylation (8).
PIP4K2C, also known as phosphatidylinositol-5-phosphate 4-kinase type-2 gamma (PI5P4Kγ), is a member of the type II PIP kinase family, and is broadly expressed with the highest levels in kidney and brain tissue (9). PIP4K2C can form a homodimer or heterodimer with its two other known family members, PIP4K2A and PIP4K2B (10). PIP4K2C is a regulator of mammalian target of rapamycin complex 1 (mTORC1) signaling, and Pip4k2c KO mice demonstrate enhanced immune responses and develop autoimmunity (11,12).
- Rameh, L.E. et al. (1997) Nature 390, 192-6.
- Zhang, X. et al. (1997) J Biol Chem 272, 17756-61.
- Ishihara, H. et al. (1996) J Biol Chem 271, 23611-4.
- Loijens, J.C. and Anderson, R.A. (1996) J Biol Chem 271, 32937-43.
- Ishihara, H. et al. (1998) J Biol Chem 273, 8741-8.
- Itoh, T. et al (1998) J. Biol. Chem. 273, 20292-20299
- Boronenkov, I.V. et al. (1998) Mol Biol Cell 9, 3547-60.
- Oude Weernink, P.A. et al. (2004) Eur J Pharmacol 500, 87-99.
- Clarke, J.H. et al. (2008) Am J Physiol Renal Physiol 295, F1422-30.
- Ciruela, A. et al. (2000) Biochem J 346 Pt 3, 587-91.
- Mackey, A.M. et al. (2014) Sci Signal 7, ra104.
- Shim, H. et al. (2016) Proc Natl Acad Sci USA 113, 7596-601.
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