ATP5O (E7F4U) Rabbit mAb #92658
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 23 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ATP5O protein.
Background
ATP synthase subunit O (ATP5O, OSCP, or ATP5PO) is a component of the mitochondrial complex V, which functions to generate ATP from ADP. ATP synthase is composed of a soluble catalytic core, F1, and a membrane-spanning proton (H+) channel, FO. ATP5O is part of the peripheral stalk linking the two structural domains. The ATP5O N-terminus directly interacts with subunit F1, while the C-terminus directly interacts with FO and is critical for the oligomycin sensitivity of the H+ channel (1,2). The expression of ATP5O is elevated in cancer-associated fibroblasts (CAFs) compared to normal fibroblasts in oral squamous cell carcinoma, reflecting the elevated oxidative phosphorylation requirements for ATP generation in these cells (3). Recent studies have shown elevated ATP5O as a biomarker for prostate and gastric cancers (4,5). Conversely, direct binding of claudin-10 (CLDN10) to ATP5O in the outer mitochondrial membrane increases ATP5O expression and acetylation, leading to disrupted mitochondria and reduced metastasis in clear cell renal cell carcinoma (ccRCC) (6).
- Joshi, S. et al. (1996) Biochemistry 35, 12094-103.
- Joshi, S. et al. (1992) J Biol Chem 267, 12860-7.
- Xiao, L. et al. (2021) BMC Cancer 21, 1329.
- Wiebringhaus, R. et al. (2021) Cancers (Basel) 13, 6036. doi: 10.3390/cancers13236036.
- Sotgia, F. and Lisanti, M.P. (2017) Oncotarget 8, 67117-67128.
- Yang, W. et al. (2022) Int J Biol Sci 18, 2329-2344.
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