IL-17A (D1X7L) Rabbit mAb (BSA and Azide Free) #90428
- WB
- F
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 17, 14 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val49 of mouse IL-17A protein.
Background
The IL-17 family of cytokines consists of IL-17A-F, and their receptors include IL-17RA-RE (1). IL-17 cytokines are produced by a variety of cell types including the Th17 subset of CD4+ T cells, as well as subsets of γδ T cells, NK cells, and NKT cells (2). IL-17A and IL-17F, the most well-studied of the IL-17 cytokines, contribute to fungal and bacterial immunity by inducing expression of proinflammatory cytokines, chemokines, and antimicrobial peptides (2). In addition, IL-17A contributes to the pathogenesis of several autoimmune diseases (3). IL-17E promotes Th2 cell responses (4). The roles of IL-17B, IL-17C, and IL-17D are less clear, however these family members also appear to have the capacity to induce proinflammatory cytokines (1,5,6). IL-17 receptors have an extracellular domain, a transmembrane domain, and a SEFIR domain. They are believed to signal as homodimers, heterodimers, or multimers through their SEFIR domain by recruiting the SEFIR domain-containing adaptor Act1 (7). Unlike most cytokines that signal through Jak/STAT pathways, IL-17 signaling results in NF-κB activation (8).
IL-17A is a cysteine-linked, homodimeric, pro-inflammatory cytokine produced by Th17 cells, a distinct CD4+ T cell lineage (9,10). IL-17A stimulates the production of the pro-inflammatory cytokines IL-1β, TNFα, and IL-6. IL-17A also induces production of the neutrophil chemoattractants IL-8, CXCL1, and CXCL6 thereby bridging adaptive and innate immunity (9,10). IL-17A is intimately involved in mucosal immunity against bacterial infections (9,11) and has a putative role in some autoimmune disorders (9,12). IL-17A effects appear to be exerted primarily through binding to one of the IL-17 receptor subunits, IL-17RA (13). IL-17 binding induces production of cytokines, chemokines, and other proteins through activation of the Erk1/2 MAP kinase, PI3K/Akt, p38, and NF-κB pathways (11,12,14). Phosphorylation of some Jaks and Stats has been observed.
- Gaffen, S.L. (2009) Nat Rev Immunol 9, 556-67.
- Iwakura, Y. et al. (2011) Immunity 34, 149-62.
- Hu, Y. et al. (2011) Ann N Y Acad Sci 1217, 60-76.
- Fort, M.M. et al. (2001) Immunity 15, 985-95.
- Yamaguchi, Y. et al. (2007) J Immunol 179, 7128-36.
- Li, H. et al. (2000) Proc Natl Acad Sci U S A 97, 773-8.
- Chang, S.H. et al. (2006) J Biol Chem 281, 35603-7.
- Shalom-Barak, T. et al. (1998) J Biol Chem 273, 27467-73.
- Kolls, J.K. and Lindén, A. (2004) Immunity 21, 467-76.
- Liang, S.C. et al. (2006) J Exp Med 203, 2271-9.
- Dubin, P.J. and Kolls, J.K. (2008) Immunol Rev 226, 160-71.
- Zrioual, S. et al. (2009) J Immunol 182, 3112-20.
- Wright, J.F. et al. (2008) J Immunol 181, 2799-805.
- Rahman, M.S. et al. (2006) J Immunol 177, 4064-71.
限制使用
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