NK1.1/CD161 (PK136) Mouse mAb (PerCP-Cy5.5® Conjugate) #88957
- F
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG2a kappa |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
Product Usage Information
For optimal flow cytometry results, we recommend 0.25 μg of antibody per test.
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:80 |
| Flow Cytometry (Live) | 1:80 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Background
CD161/KLRB1 (Killer cell lectin-like receptor subfamily B member 1, also known as CLEC5B and NKR-P1A) is a type II transmembrane protein that is expressed on the majority of Natural Killer (NK) cells, NK T cells, and some T lymphocytes (1). CD161/KLRB1 is also expressed on Th17 cells, promotes their generation, and modulates their function (2). Engagement with its ligand lectin-like transcript 1 (LLT1) inhibits NK cell function, while LLT1 and CD161/KLRB1 interaction in the presence of a TCR signal enhances IFN-gamma production by T cells (3,4). There are several different CD161 isoforms in rodents and some function as activating receptors as well (5,6).
There is a family of Klrb1 genes in rodents (7). PK136 antibody recognizes a specific epitope on mouse KLRB1b/c, also referred to as NK1.1, and is commonly used for detection of mouse NK cells in certain mouse strains (CE, B6, NZB, C58, Ma/My, ST, SJL, FVB). However, the epitope is absent in other mouse strains (BALB/c, AKR, CBA, C3H, DBA, 129) (8).
- Lanier, L.L. et al. (1994) J Immunol 153, 2417-28.
- Bai, A. et al. (2014) J Immunol 193, 3366-77.
- Aldemir, H. et al. (2005) J Immunol 175, 7791-5.
- Rosen, D.B. et al. (2005) J Immunol 175, 7796-9.
- Carlyle, J.R. et al. (2006) J Immunol 176, 7511-24.
- Kirkham, C.L. and Carlyle, J.R. (2014) Front Immunol 5, 214.
- Kirkham, C.L. and Carlyle, J.R. (2014) Front Immunol 5, 214.
- Carlyle, J.R. et al. (2006) J Immunol 176, 7511-24.
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