Render Target: SSR
Render Timestamp: 2024-08-29T21:51:27.063Z
Commit: 419142f0105700b709b94a8ebde4734bae2c62a3
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PDP - Template Name: Monoclonal Antibody (Alexa Fluor Conjugate)
PDP - Template ID: *******c8ce56b
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

G3BP1 (E9G1M) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #83279

Filter:
  • IF

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated G3BP1 (E9G1M) XP® Rabbit mAb #61559.

    Product Usage Information

    Application Dilution
    Immunofluorescence (Immunocytochemistry) 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    G3BP1 (E9G1M) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) recognizes endogenous levels of total G3BP1 protein. This antibody does not cross-react with G3BP2 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val218 of human G3BP1 protein.

    Background

    Ras GTPase-activating protein-binding protein 1, also known as GAP SH3 domain-binding protein 1 (G3BP1), was identified as a protein that interacts with the SH3 domain of Ras GTPase-activating protein (RasGap) (1). G3BP1 is involved in the regulation of multiple cellular processes, including mRNA decay and inhibition of translation initiation (2). Furthermore, G3BP1 is essential for the assembly of stress granules (SGs) and functions as an SG-nucleating protein (3). Research studies show that arginine demethylation of G3BP1 promotes SG assembly during oxidative stress (4).

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.