GP130 (E6X8S) Rabbit mAb #76689
- WB
- IP
- IF
- F
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 130 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 - 1:400 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln775 of human GP130 protein.
Background
GP130 is a signal-transducing subunit shared by the receptors for the interleukin-6 (IL-6) family of cytokines (1-3). The binding of a ligand to its receptor induces the dimerization of GP130, leading to activation of the Jak tyrosine kinase and to tyrosine phosphorylation of GP130. These events lead to the activation of multiple signal-transduction pathways, such as the Stat, Ras-MAPK, and PI3 kinase pathways, whose activation is controlled by distinct regions of GP130 (4-7).
- Taga, T. (1996) J. Neurochem. 67, 1-10.
- Taga, T. (1997) Ann. Med. 29, 63-72.
- Taga, T. and Kishimoto, T. (1997) Annu. Rev. Immunol. 15, 797-819.
- Heinrich, P.C. et al. (1998) Biochem. J. 334, 297-314.
- Ihle, J.N. et al. (1994) Trends Biochem. Sci. 19, 222-227.
- Kurth, I. et al. (1999) J. Immunol. 162, 1480-1487.
- Paonessa, G. et al. (1995) EMBO J. 14, 1942-1951.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
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