V5-Tag (D3H8Q) Rabbit mAb (Sepharose^® Bead Conjugate) #67476

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Supporting Data

REACTIVITY	All
SENSITIVITY	Transfected Only
MW (kDa)
Source/Isotype	Rabbit IgG

Application Key:

IP-Immunoprecipitation

Species Cross-Reactivity Key:

All-All Species Expected

Product Information

Product Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose^® beads. V5-Tag (D3H8Q) Rabbit mAb (Sepharose^® Bead Conjugate) is useful for immunoprecipitation assays. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated V5-Tag (D3H8Q) Rabbit mAb #13202.

Product Usage Information

Application	Dilution
Immunoprecipitation	1:20

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

V5-Tag (D3H8Q) Rabbit mAb (Sepharose^® Bead Conjugate) recognizes transfected levels of recombinant protein containing the V5 epitope tag.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic V5-tag peptide.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

The V5-tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family (1). The V5-tag can be used to detect expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.

Hanke, T. et al. (1995) J Virol Methods 53, 149-56.

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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V5-Tag (D3H8Q) Rabbit mAb (Sepharose® Bead Conjugate) #67476