BCAP (E1U9X) Rabbit mAb #63633
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 105, 98, 85, 78 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human BCAP protein.
Background
B cell adaptor for PI3K (BCAP) is a multidomain-containing signaling adaptor that is expressed by myeloid cells, B cells, and NK cells (1,2). Two major BCAP protein isoforms that differ significantly in size, BCAP-L and BCAP-S, have been shown to be expressed by macrophages, B cells, and NK cells (1,2). Although it remains unclear if there is a differential function for these isoforms, both BCAP-L and BCAP-S undergo robust tyrosine phosphorylation by multiple tyrosine kinases in response to B cell receptor and Toll-like receptor engagement, which promotes association of BCAP with multiple SH2- and SH3-domain-containing proteins (1,3,4). In the context of B cells, research studies have demonstrated that phosphorylation of the YXXM motifs within BCAP are critical for the recruitment and activation of PI3K to drive B cell proliferation (4,5). Macrophages, however, utilize the BCAP/PI3K signaling module to restrain proinflammatory functions in response to infection (3,6).
- Okada, T. et al. (2000) Immunity 13, 817-27.
- MacFarlane, A.W. et al. (2008) Blood 112, 131-40.
- Ni, M. et al. (2012) Proc Natl Acad Sci U S A 109, 267-72.
- Inabe, K. and Kurosaki, T. (2002) Blood 99, 584-9.
- Yamazaki, T. et al. (2002) J Exp Med 195, 535-45.
- Troutman, T.D. et al. (2012) Proc Natl Acad Sci U S A 109, 273-8.
限制使用
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