Oligodendrocyte Marker Antibody Sampler Kit

The Oligodendrocyte Marker Antibody Sampler Kit provides an economical means of detecting proteins identified as oligodendrocyte markers by immunofluorescence and western blot. This kit includes enough primary antibodies to perform at least twenty IF tests or two western blot experiments per primary antibody.

Oligodendrocytes are the myelinating glial cells of the central nervous system (CNS) (1). Myelin basic protein (MBP) is an abundant CNS myelin membrane protein that plays an important role in nerve myelination. MBP helps to adhere the cytoplasmic leaflets of adjacent oligodendrocyte membranes to one another (2). CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase) is an enzyme highly expressed in oligodendrocytes and accounts for roughly 4% of the total myelin protein in the CNS (3). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth (4). Myelin proteolipid protein (PLP1) corresponds to the majority of myelin proteins in the CNS, providing support to axons and modulating axonal growth (5). Myelin-associated glycoprotein (MAG) is localized in oligodendroglial membranes of myelin sheaths where it plays a role in interaction between axons and glia, and has been shown to promote axonal protective effects (6,7). Chondroitin sulfate proteoglycan 4 (CSP4, NG2) is a type I membrane glycoprotein expressed by oligodendroglial precursor cells (OPCs) that plays a role in cell attachment and migration (8). SRY-box 10 (Sox10) is a high-mobility group transcription factor expressed throughout oligodendrocyte development required for myelin gene expression (9,10). Myelin-oligodendrocyte glycoprotein (MOG) is a type I membrane bound glycoprotein of the immunoglobulin superfamily that is enriched in the outer lamella of the myelin sheath (11). Olig2 is a basic helix–loop–helix (bHLH) transcription factor necessary for oligodendrocyte and motor neuron differentiation and development in the CNS (12,13). Olig2 has been shown to be a useful marker for primary and mature oligodendrocytes and is universally expressed in diffuse gliomas (14,15).