CD28 (CD28.2) Mouse mAb (PerCP-Cy5.5® Conjugate) #25544
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 kappa |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Live) | 1:20 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Background
CD28 is a transmembrane glycoprotein expressed by T cells as well as some other hematopoietic cells (1, 2). T cell activation requires T cell receptor (TCR) recognition of antigen presented in the context of MHC molecules. CD28 acts as a T cell costimulatory receptor, and interaction of CD28 with its ligands CD80 or CD86 provides the second signal required for naïve T cell activation (3-5). Activation of naïve T cells in the absence of CD28 stimulation can result in a state of T cell anergy, or unresponsiveness (3). CD28 signals through cytoplasmic phospho-tyrosine motifs that bind several SH2 or SH3 domain-containing proteins involved in T cell activation (2). Recently, CD28 was demonstrated to be a preferred target of PD-1-mediated dephosphorylation. Consistently, CD28 expression was required for T cell proliferation following PD-1 blockade and CD28 stimulation was required for effective anti-PD-1 cancer immunotherapy in mice (6, 7). Several CD28 isoforms are produced by alternative splicing (8).
- Aruffo, A. and Seed, B. (1987) Proc Natl Acad Sci U S A 84, 8573-7.
- Esensten, J.H. et al. (2016) Immunity 44, 973-88.
- Harding, F.A. et al. (1992) Nature 356, 607-9.
- Azuma, M. et al. (1993) Nature 366, 76-9.
- Linsley, P.S. et al. (1990) Proc Natl Acad Sci U S A 87, 5031-5.
- Hui, E. et al. (2017) Science 355, 1428-1433.
- Kamphorst, A.O. et al. (2017) Science 355, 1423-1427.
- Magistrelli, G. et al. (1999) Biochem Biophys Res Commun 259, 34-7.
限制使用
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