Revision 7

#2146

Store at -20C

β-Tubulin Antibody
CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IHC-P, IF-IC, FC-FP

Reactivity:
H M R Mk Z B

Sensitivity:
Endogenous

MW (kDa):
55

Source/Isotype:
Rabbit

UniProt ID:
#P07437

Entrez-Gene Id:
203068

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:75 - 1:300
Immunofluorescence (Immunocytochemistry) 1:50
Flow Cytometry (Fixed/Permeabilized) 1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

The β-Tubulin Antibody detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin.

Species predicted to react based on 100% sequence homology

Xenopus

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human β-tubulin. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

Background References

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: Human M: Mouse R: Rat Mk: Monkey Z: Zebrafish B: Bovine

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品 ， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 7

#2146

β-Tubulin Antibody

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using β-tubulin Antibody.
Western Blotting Image 1: β-Tubulin Antibody
Western blot analysis of extracts from CAD and C6 cells using β-Tubulin Antibody.
Western Blotting Image 2: β-Tubulin Antibody
Western blot analysis of recombinant alpha-tubulin and beta-tubulin GST-fusion proteins and extracts from CAD cells using α-Tubulin Antibody #2144(left), β-Tubulin Antibody (middle) and GST Antibody #2622 (right).
Western Blotting Image 3: β-Tubulin Antibody
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 7

#2146

β-Tubulin Antibody

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using β-Tubulin Antibody.
Immunohistochemistry Image 1: β-Tubulin Antibody
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using β-Tubulin Antibody.
Immunohistochemistry Image 2: β-Tubulin Antibody
Immunohistochemical analysis of paraffin-embedded human MALToma, using β-Tubulin Antibody.
Immunohistochemistry Image 3: β-Tubulin Antibody
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 7

#2146

β-Tubulin Antibody

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Tubulin Antibody in the presence of control peptide (left) or β-Tubulin Blocking Peptide #1032 (right).
Immunohistochemistry Image 4: β-Tubulin Antibody
Confocal microscopic images of NIH/3T3 cells showing cytoskeletal stain with β-Tubulin Antibody (A) compared to an isotype control (B).
Immunofluorescence Image 1: β-Tubulin Antibody
Flow cytometric analysis of NIH/3T3 cells using β-Tubulin Antibody (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Flow Cytometry Image 1: β-Tubulin Antibody
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.