Name: PathScan ® Phospho-Rb (Ser780) Sandwich ELISA Kit
Price: 613.00 USD
Availability: InStock

Supporting Data

REACTIVITY

H

Application Key:

ELISA-ELISA

Species Cross-Reactivity Key:

H-Human

Product Information

Product Description

PathScan^® Phospho-Rb (Ser780) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of phospho-Rb (Ser780) protein. A phospho-Rb (Ser780) specific antibody has been coated onto the microwells. After incubation with cell lysates, phospho-Rb (Ser780) protein is captured by the coated antibody. Following extensive washing, a Rb mouse mAb is added to detect the captured phospho-Rb protein. HRP-linked Anti-Mouse IgG is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of phospho-Rb (Ser780) protein.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

PathScan^® Phospho-Rb (Ser780) Sandwich ELISA Kit detects endogenous levels of Rb protein when phosphorylated at Ser780, as shown in figure 1. The kit sensitivity is shown in Figure 2. Phosphatase treatment, as well as dot blot analysis (data not shown) using specific phosphopeptides and non-phosphopeptides, was used to confirm the phospho-specificity of the antibodies provided in this kit. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

Background

The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).

Database Links

UniProt ID: P06400

Entrez-Gene Id: 5925

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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PathScan is a registered trademark of Cell Signaling Technology, Inc.

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PathScan® Phospho-Rb (Ser780) Sandwich ELISA Kit #13016