VEGF Receptor 2 (D5B1) Rabbit mAb (Alexa Fluor® 488 Conjugate) #12687
- F
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Description
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein containing the carboxy-terminal 150 amino acid residues of human VEGF receptor 2 protein.
Background
Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).
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- Dougher-Vermazen, M. et al. (1994) <cite>Biochem Biophys Res Commun</cite> 205, 728-38.
- Kroll, J. and Waltenberger, J. (1997) <cite>J Biol Chem</cite> 272, 32521-7.
- Takahashi, T. et al. (2001) <cite>EMBO J</cite> 20, 2768-78.
- Holmqvist, K. et al. (2004) <cite>J Biol Chem</cite> 279, 22267-75.
- Karkkainen, M.J. and Petrova, T.V. (2000) <cite>Oncogene</cite> 19, 5598-605.
- Rahimi, N. et al. (2000) <cite>J Biol Chem</cite> 275, 16986-92.
- Claesson-Welsh, L. (2003) <cite>Biochem Soc Trans</cite> 31, 20-4.
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