CD86/B7-2 (GL-1) Rat mAb (PE-Cy7® Conjugate) #11527
- F
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rat IgG2a kappa |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
Product Usage Information
For optimal flow cytometry results, we recommend 0.25 μg of antibody per test. A slight precipitate may be present, but will not interfere with the antibody performance. If precipitates are present, centrifuge the tube at 6,000xg for 10-30 sec. Draw off the supernatant and place into a light protective vial.
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:80 |
| Flow Cytometry (Live) | 1:80 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Background
CD80 (B7-1, BB1) and CD86 (B7-2, B70) are members of the B7 family of cell surface ligands that regulate T cell activation and immune responses. CD80 is expressed on activated antigen presenting cells, including dendritic cells, B cells, monocytes, and macrophages. CD86 is expressed on resting monocytes, dendritic cells, activated B lymphocytes, and can be further upregulated in the presence of inflammation (1-3). CD80 and CD86 are ligands for CD28, which functions as a T cell costimulatory receptor. Interaction of CD28 with CD80 or CD86 provides the second signal required for naïve T cell activation, T cell proliferation, and acquisition of effector functions (3-7). Alternatively, CD80 and CD86 also act as ligands to CTLA-4, which results in the downregulation of T cell activity (3,7-9).
The GL-1 antibody is used as a marker for CD86 expression on B cells, macrophages, and dendritic cells.
- Freeman, G.J. et al. (1989) J Immunol 143, 2714-22.
- Azuma, M. et al. (1993) Nature 366, 76-9.
- Adams, A.B. et al. (2016) J Immunol 197, 2045-50.
- Linsley, P.S. et al. (1990) Proc Natl Acad Sci U S A 87, 5031-5.
- Gimmi, C.D. et al. (1991) Proc Natl Acad Sci U S A 88, 6575-9.
- Harding, F.A. et al. (1992) Nature 356, 607-9.
- Collins, A.V. et al. (2002) Immunity 17, 201-10.
- Linsley, P.S. et al. (1991) J Exp Med 174, 561-9.
- Krummel, M.F. and Allison, J.P. (1995) J Exp Med 182, 459-65.
限制使用
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