Revision 1

#58992

Store at RT

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
PTMScan

Product Usage Information

Use with a Peptide Desalting C18 StageTip for processing peptide samples prior to liquid chromatography-mass spectrometry (LCMS). Dimensions of microcentrifuge tubes and centrifuge devices can vary, and we recommend a microcentrifuge tube that can accommodate the inner diameter of the adaptor and a centrifuge that can accommodate the tube, adaptor, and StageTip with a rotor lid. Below are examples of equipment we have tested that fit the provided adaptor.

Recommended equipment (not provided):
Posi-Click 1.5 mL graduated microcentrifuge tube (Thomas Scientific)
Peptide Desalting C18 StageTip (Cell Signaling Technology, #45943)
MiniSpin Centrifuge with F-45-12-11 rotor (Eppendorf)

1) Remove the lid from a clean 1.5 mL microcentrifuge tube.

2) Insert an adaptor inside the microcentrifuge tube.

3) Insert a Peptide Desalting C18 StageTip through the central hole in the adaptor. The StageTip should sit easily inside the adaptor. There should be enough space between the bottom of the microcentrifuge tube and the bottom of the StageTip to accommodate at least 100 μL of liquid. Users can proceed with peptide desalting.

4) After experiments are finished, users can wipe clean the adaptor with 70% ethanol and air dry it if necessary. DO NOT use detergent- or soap-containing solutions to clean the adaptor. Users can store the adaptor for re-use at room temperature in a closed bag or box to minimize dust exposure.

Storage

Store at room temperature.

Description

The Peptide Desalting C18 StageTip Adaptor is a reusable option to vertically position a C18 StageTip inside a microcentrifuge tube, allowing for removal of wash and waste solutions and for collection of desalted peptide elutions.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

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专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品 , (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

CST Logo
A Peptide Desalting C18 StageTip Adaptor can be inserted into a 1.5 mL microcentrifuge tube (not provided). A Peptide Desalting C18 StageTip #45943 can then be placed through the central hole of the adaptor, such that solutions can pass through StageTip by centrifugation and collect in the bottom of the microcentrifuge tube.
undefined Image 1: Peptide Desalting C18 StageTip Adaptor
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.