Revision 5
#33036
Store at -20C
877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
PTMScan
Source/Isotype:
E. coli
UniProt ID:
#P00778
Product Usage Information
PTMScan® Wild Type Alpha-Lytic Protease (WaLP) is provided for use with Cell Signaling Technology's patented PTMScan® protocol in the initial protein digestion step. Samples in urea lysis buffer need to be diluted to 2M or less urea prior to addition of WaLP. Dilute the 0.4 mg/ml stock 1:100 for final digest and incubate overnight at 37°C. Aliquot and store at -20ºC. Consult the specific PTMScan® kit and protocol for more details on the appropriate protease before digesting any protein samples.
Storage
Species predicted to react based on 100% sequence homology
Source / Purification
Description
Background
Background References
- Whitaker, DR. The alpha-Lytic Protease. Methods in Enzymology, 19, 599-613
- Sohl, JL, et al., , IntramolecularChaperones and Protein Folding, Landes Company, Austin TX (1995) ISBN 978-1570592928 pp. 61-83
- Bauer, C.A. et al. (1981) Eur J Biochem 120, 289-94.
- Bachovchin, W.W. et al. (1981) Proc Natl Acad Sci U S A 78, 7323-6.
- Olson, M.O. et al. (1970) Nature 228, 438-42.
- Meyer, J.G. et al. (2014) Mol Cell Proteomics 13, 823-35.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PTMScan is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
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