Tox/Tox2 (E6G5O) Rabbit mAb recognizes endogenous levels of total Tox and Tox2 proteins. This antibody does not cross-react with Tox3 or Tox4 proteins. This antibody cross-reacts with an unidentified protein of 50 kDa in some murine cell extracts.

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln435 of human Tox protein.

Thymocyte selection-associated high mobility group box protein (Tox) is a DNA-binding nuclear factor and member of the evolutionarily conserved high-motility group (HMG)-box superfamily. Tox also defines a small subfamily of proteins that include Tox2, Tox3, and Tox4, all of which are highly conserved in vertebrate species but have unique tissue expression patterns and functions (1,2).

Tox plays a key role in T cell development in the thymus during positive selection (3-5). A study in Tox-deficient mice also revealed a requirement for Tox in CD4 T cell and NK cell lineage development, including NKT cells, FoxP3+ T regulatory T cells, and lymphoid tissue-inducer (LTi) cells (6-8). Although Tox expression is primarily restricted to developing immune cells in normal tissues, Tox is induced by high antigen stimulation during chronic viral infection or cancer, regulating T cell persistence and exhaustion (9-12). Tox has also been shown to be aberrantly expressed in cutaneous T cell lymphomas (13-14).