Revision 5

#72804

Store at -20C

TAZ (E9J5A) XP® Rabbit mAb
CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP, IHC-Bond, IHC-P, ChIP

Reactivity:
H M Mk

Sensitivity:
Endogenous

MW (kDa):
55

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q9GZV5

Entrez-Gene Id:
25937

Product Usage Information

For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 10^6 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
IHC Leica Bond 1:100 - 1:400
Immunohistochemistry (Paraffin) 1:200 - 1:800
Chromatin IP 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #49756.

Specificity/Sensitivity

TAZ (E9J5A) XP® Rabbit mAb recognizes endogenous levels of total TAZ protein. This antibody does not cross-react with YAP protein. Species cross-reactivity for IHC-P is human only.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinantly expressed full-length human TAZ protein.

Background

TAZ is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 proteins (1). TAZ shares homology with the WW domain of Yes-associated protein (YAP) (1). TAZ is proposed to modulate the switch between proliferation and differentiation of mesenchymal stem cells (MSC) via interaction with transcription factors Runx2 and PPARγ. This process is critical to normal tissue development and the prevention of tumor formation. Due to its role in determination of MSC fate, TAZ may have clinical relevance to several human diseases caused by an imbalance of MSC differentiation (2,3). TAZ is negatively regulated via phosphorylation by LATS1/2, core kinases in the Hippo signaling pathway that controls stem cell development, tissue growth and tumor development (4).

Background References

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin) ChIP: Chromatin IP

Cross-Reactivity Key

H: Human M: Mouse Mk: Monkey

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStain is a registered trademark of Cell Signaling Technology, Inc.

SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品 ， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

#72804

TAZ (E9J5A) XP® Rabbit mAb

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using TAZ (E9J5A) XP® Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 1: TAZ (E9J5A) XP<sup>®</sup> Rabbit mAb
Immunoprecipitation of TAZ protein from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is TAZ (E9J5A) XP® Rabbit mAb. Western blot analysis was performed using TAZ (D3I6D) Rabbit mAb #70148. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.
Immunoprecipitation Image 1: TAZ (E9J5A) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human ovarian clear cell carcinoma using TAZ (E9J5A) XP® Rabbit mAb performed on the Leica® BOND™ Rx.
Immunohistochemistry Image 1: TAZ (E9J5A) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

#72804

TAZ (E9J5A) XP® Rabbit mAb

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using TAZ (E9J5A) XP® Rabbit mAb performed on the Leica® BOND™ Rx.
Immunohistochemistry Image 2: TAZ (E9J5A) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded BEN cell pellet, untransfected (left, YAP/TAZ low-expressing), YAP-transfected (middle), or TAZ-transfected (right), using TAZ (E9J5A) XP® Rabbit mAb (top) or YAP (D8H1X) XP® Rabbit mAb #14074 (bottom).
Immunohistochemistry Image 1: TAZ (E9J5A) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using TAZ (E9J5A) XP® Rabbit mAb.
Immunohistochemistry Image 2: TAZ (E9J5A) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

#72804

TAZ (E9J5A) XP® Rabbit mAb

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using TAZ (E9J5A) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 3: TAZ (E9J5A) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using TAZ (E9J5A) XP® Rabbit mAb.
Immunohistochemistry Image 4: TAZ (E9J5A) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using TAZ (E9J5A) XP® Rabbit mAb.
Immunohistochemistry Image 5: TAZ (E9J5A) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

#72804

TAZ (E9J5A) XP® Rabbit mAb

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma (left), colon carcinoma case 1 (middle), or colon carcinoma case 2 (right), using TAZ (E9J5A) XP® Rabbit mAb (top) or YAP (D8H1X) XP® Rabbit mAb #14074 (bottom).
Immunohistochemistry Image 6: TAZ (E9J5A) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded SK-MEL-28 cell pellet (left, positive) or RL cell pellet (right, negative) using TAZ (E9J5A) XP® Rabbit mAb.
Immunohistochemistry Image 7: TAZ (E9J5A) XP<sup>®</sup> Rabbit mAb
Chromatin immunoprecipitations were performed with cross-linked chromatin from untreated NCI-H2052 cells and either TAZ (E9J5A) XP® Rabbit mAb #72804 or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CTGF Promoter Primers #14927, human SMYD3 Intron 2 primers, SimpleChIP® Human CTGF Upstream Primers #14928. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Chromatin Immunoprecipitation Image 1: TAZ (E9J5A) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.