Revision 1
#93005
Store at -20C
877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP, IF-F, IF-IC, FC-FP, FC-L
Reactivity:
M
Sensitivity:
Endogenous
MW (kDa):
20-30
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q9ET35
Entrez-Gene Id:
57916
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Frozen) | 1:50 - 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:100 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 - 1:200 |
| Flow Cytometry (Live) | 1:50 - 1:200 |
Storage
Specificity/Sensitivity
Source / Purification
Background
Background References
- von Bülow, G.U. et al. (2000) Mamm Genome 11, 628-32.
- Mantchev, G.T. et al. (2007) J Immunol 179, 2282-8.
- Tsuji, S. et al. (2011) Blood 118, 5832-9.
- Wang, H. et al. (2001) Nat Immunol 2, 632-7.
- Chang, S.K. et al. (2006) Blood 108, 2687-94.
- Chang, S.K. et al. (2008) J Immunol 180, 7394-403.
- Wu, Y. et al. (2000) J Biol Chem 275, 35478-85.
- Yu, G. et al. (2000) Nat Immunol 1, 252-6.
- Hymowitz, S.G. et al. (2005) J Biol Chem 280, 7218-27.
- Xia, X.Z. et al. (2000) J Exp Med 192, 137-43.
- von Bülow, G.U. and Bram, R.J. (1997) Science 278, 138-41.
- Ozcan, E. et al. (2009) J Allergy Clin Immunol 123, 1277-86.e5.
- Seshasayee, D. et al. (2003) Immunity 18, 279-88.
- Ou, X. et al. (2012) Proc Natl Acad Sci U S A 109, 15401-6.
- He, B. et al. (2010) Nat Immunol 11, 836-45.
- Castigli, E. et al. (2005) Nat Genet 37, 829-34.
- Moura, R.A. et al. (2013) J Rheumatol 40, 1293-302.
- Abo-Elfadl, M.T. et al. (2020) Cytokine 125, 154790.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized) FC-L: Flow Cytometry (Live)
Cross-Reactivity Key
M: Mouse
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
限制使用
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Revision 1
Revision 1
