Revision 1

#55851

Store at -20C

Siglec-7 (F4O1S) Rabbit mAb
CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
80-100

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q9Y286

Entrez-Gene Id:
27036

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

Siglec-7 (F4O1S) Rabbit mAb recognizes endogenous levels of total Siglec-7 protein. This antibody detects an 18 kDa band of unknown origin in some cell lines.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met395 of human Siglec-7 protein.

Background

Siglec-7 (Sialic acid-binding Ig-like lectin 7, AIRM1) is a member of the CD33-related Siglec family, a subset of the immunoglobulin superfamily of cell surface proteins (1-3). Siglec-7 is found in human immune cells such as natural killer (NK) cells, monocytes, and neutrophils (1,2). Siglec-7 acts as an immune inhibitory receptor via its intracellular Tyrosine-based Inhibitory Motif (ITIM) (1-5). Downregulation of cell surface Siglec-7 is an early marker of NK cell dysfunction, which can be associated with chronic viral infection or impaired anti-tumor activity (4).

Background References

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

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Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.