Revision 1

#50832

Store at -20C

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP

Reactivity:
H M R

Sensitivity:
Endogenous

MW (kDa):
46, 52, 66

Source/Isotype:
Rabbit IgG

UniProt ID:
#P29353

Entrez-Gene Id:
6464

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Specificity/Sensitivity

Shc (E6I4B) Rabbit mAb recognizes endogenous levels of total Shc protein. This antibody detects three isoforms at 46, 52, and 66 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro320 of human SHC1 protein.

Background

SHC-transforming protein 1 (SHC1) is an adaptor protein to targets of activated tyrosine kinases. SHC1 is ubiquitously expressed and the gene is expressed in 2 transcripts, resulting in 3 isoforms with distinct functions and subcellular locations (1). The phosphorylated p46 and p52 isoforms couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex. The third isoform, p66, acts as a downstream target of the tumor suppressor p53 and is thereby implicated in oxidative stress response, tumor progression, and cancer cell proliferation (2,3). SHC1 has been identified to have prognostic and diagnostic value in multiple cancers, making it a potential biomarker (4).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: Human M: Mouse R: Rat

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意,否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件,除非书 CST 的合法授书代表以书面形式书独接受, 否书均被拒书,并且无效。

专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品 , (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from Hep G2 and Jurkat cells using Shc (E6I4B) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 1: Shc (E6I4B) Rabbit mAb
Immunoprecipitation of Shc protein from Hep G2 cells. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Shc (E6I4B) Rabbit mAb. Western blot analysis was performed using Shc (E6I4B) Rabbit mAb. Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb #45262 was used as a secondary antibody.
Immunoprecipitation Image 1: Shc (E6I4B) Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.