Pyruvate Carboxylase Antibody #66470
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 130 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Pyruvate Carboxylase Antibody recognizes endogenous levels of total pyruvate carboxylase protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human pyruvate carboxylase protein. Antibodies are purified by peptide affinity chromatography.
Background
Pyruvate carboxylase (PC) catalyzes the carboxylation of pyruvate to oxaloacetate in an anaplerotic pathway to replenish TCA cycle intermediates. It is also critical in regulating gluconeogenesis in the liver (1). Studies show that the increase of PC flux contributes to the induction of hepatic gluconeogenesis by glucagon (2). In addition, high-fat diet is shown to promote hepatic glucose uptake and induce PC activity in the liver (3). Loss of hepatic PC prevents diet-induced hyperglycemia and insulin resistance. Furthermore, this enzyme is also required for antioxidant defenses (1).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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