Revision 1

#69387

Store at -20C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP

Reactivity:
H M R

Sensitivity:
Endogenous

MW (kDa):
101

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q99JP0

Entrez-Gene Id:
225028

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

MAP4K3 (E9L1J) Rabbit mAb recognizes endogenous levels of total MAP4K3 protein. A band of unknown identity is detected at 48 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His404 of human MAP4K3 protein.

Background

The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by growth factors and nutrients including amino acids (1,2). MAP4K3 is a mediator between nutrient signal and mTORC1 (1). Research studies suggest that amino acid sufficiency leads to the phosphorylation of Ser170 on MAP4K3, which activates mTORC1 (3).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

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Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

CST Logo
Western blot analysis of extracts from various cell lines using MAP4K3 (E9L1J) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of MAP4K3 protein in HH, REC-1, BA/F3, and EML cells is consistent with the predicted expression pattern.
Western Blotting Image 1: MAP4K3 (E9L1J) Rabbit mAb
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human MAP4K3 (hMAP4K3-Myc/DDK; +) or Myc/DDK-tagged full-length mouse MAP4K3 (mMAP4K3-Myc/DDK; +), using MAP4K3 (E9L1J) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 2: MAP4K3 (E9L1J) Rabbit mAb
Immunoprecipitation of MAP4K3 protein from MCF7 extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MAP4K3 (E9L1J) Rabbit mAb. Western blot analysis was performed using MAP4K3 (E9L1J) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Immunoprecipitation Image 1: MAP4K3 (E9L1J) Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.