MAdCAM-1 (E1V8F) Rabbit mAb recognizes transfected levels of total MAdCAM-1 protein by western blot and endogenous levels of total MAdCAM-1 protein by immunohistochemistry. The observed molecular weight of 60-85 kDa differs from the predicted molecular weight of 40 kDa due to glycosylation.

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAdCAM-1 protein.

Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) is a glycoprotein expressed by endothelial cells at venules of Peyer's patches, intestinal lamina propria, and mesenteric lymph nodes (1). MAdCAM-1 contributes to lymphocyte homing to these tissues, serving as a ligand for α4β7, L-Selectin, and, to a lesser extent, α4β1 (2-6). Expression of MAdCAM-1 is upregulated in patients with inflammatory bowel disease, and blocking MAdCAM-1 reduces colon inflammation by disrupting lymphocyte homing to the intestinal tract (7). In ulcerative colitis, disease activity may be a result of the L-Selectin ligand carbohydrates attached to MAdCAM-1 by N-acetylglucosamine-6-O-sulfotransferase-1 (GlcNAc6ST-1) rather than the expression levels of MAdCAM-1 alone (4).