R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
IMPDH2 (E2Q1Z) Rabbit mAb #57068
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
IMPDH2 (E2Q1Z) Rabbit mAb recognizes endogenous levels of total IMPDH2 protein. This antibody does not cross-react with IMPDH1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala419 of human IMPDH2 protein.
Background
Inosine-5'-monophosphate dehydrogenase (IMPDH) catalyzes the conversion of IMP to XMP, the first and rate-limiting step of guanine nucleotide biosynthesis (1). The isozymes IMPDH1 and IMPDH2 are encoded by two distinct genes (2). Research studies show that IMPDH2 is overexpressed in glioblastoma. In glioblastoma, IMPDH2-mediated GTP biosynthesis is essential for rRNA and tRNA synthesis and the formation of enlarged and irregular nucleoli. In addition, IMPDH1 and IMPDH2 double knockout leads to significant inhibition of tumor growth in an orthotopic glioblastoma model (3). Furthermore, IMPDH1 and IMPDH2 expression is upregulated in small cell lung cancer cells with low expression of ASCL1 transcription factor (ASCL1Low). Suppression of IMPDH activity inhibits ASCL1Low small cell lung cancer growth (4).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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