Revision 1
#45586
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP, IHC-P
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
32, 42
Source/Isotype:
Rabbit IgG
UniProt ID:
#O00214
Entrez-Gene Id:
3964
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
Specificity/Sensitivity
Source / Purification
Background
Galectin-8 (Gal-8; gene name LGALS8), which belongs to the group of tandem-repeat galectins, is important in adaptive and innate immunity with significant roles in host defense as well as tumor progression (reviewed in 2,3). Extracellular galectin-8 promotes cell adhesion through interaction with several cell surface proteins, including integrins (4,5), CD44 (6), CD45 (7), and CD166 (8). Intracellular galectin-8 binds to farnesylated K-Ras4B, regulating MAPK signaling (9). It also exerts a role in xenophagy by binding to glycans exposed on bacterial pathogens and recruiting NDP52 for destruction by autophagy, thereby restricting bacterial proliferation (10,11). Abundant expression of galectin-8 has been observed in various cancer types and, in some cases, associated with poor prognosis (12-14).
Background References
- Johannes, L. et al. (2018) J Cell Sci 131, jcs208884. doi: 10.1242/jcs.208884.
- Purić, E. et al. (2024) Med Res Rev, doi: 10.1002/med.22041.
- Zick, Y. (2022) Biochem Soc Trans 50, 135-149.
- Cárcamo, C. et al. (2006) Exp Cell Res 312, 374-86.
- Hadari, Y.R. et al. (2000) J Cell Sci 113 ( Pt 13), 2385-97.
- Eshkar Sebban, L. et al. (2007) J Immunol 179, 1225-35.
- Tribulatti, M.V. et al. (2009) J Leukoc Biol 86, 371-80.
- Delgado, V.M. et al. (2011) FASEB J 25, 242-54.
- Meinohl, C. et al. (2019) Cancers (Basel) 12, 30. doi: 10.3390/cancers12010030.
- Thurston, T.L. et al. (2012) Nature 482, 414-8.
- Kim, B.W. et al. (2013) Nat Commun 4, 1613.
- Wang, Y. et al. (2023) Am J Cancer Res 13, 2517-2529.
- Gentilini, L.D. et al. (2017) Oncotarget 8, 44654-44668.
- Elola, M.T. et al. (2014) Histol Histopathol 29, 1093-105.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
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