Revision 3

#61559

Store at -20C

G3BP1 (E9G1M) XP® Rabbit mAb
CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP, IHC-P, IF-IC

Reactivity:
H Mk

Sensitivity:
Endogenous

MW (kDa):
68

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q13283

Entrez-Gene Id:
10146

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunohistochemistry (Paraffin) 1:2500 - 1:10000
Immunofluorescence (Immunocytochemistry) 1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

G3BP1 (E9G1M) XP® Rabbit mAb recognizes endogenous levels of total G3BP1 protein. This antibody does not cross-react with G3BP2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val218 of human G3BP1 protein.

Background

Ras GTPase-activating protein-binding protein 1, also known as GAP SH3 domain-binding protein 1 (G3BP1), was identified as a protein that interacts with the SH3 domain of Ras GTPase-activating protein (RasGap) (1). G3BP1 is involved in the regulation of multiple cellular processes, including mRNA decay and inhibition of translation initiation (2). Furthermore, G3BP1 is essential for the assembly of stress granules (SGs) and functions as an SG-nucleating protein (3). Research studies show that arginine demethylation of G3BP1 promotes SG assembly during oxidative stress (4).

Background References

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品 ， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

#61559

G3BP1 (E9G1M) XP® Rabbit mAb

CST Logo
Western blot analysis of extracts from various cell lines using G3BP1 (E9G1M) XP® Rabbit mAb.
Western Blotting Image 1: G3BP1 (E9G1M) XP<sup>®</sup> Rabbit mAb
Western blot analysis of extracts from 293T cells, either wild type (+/+) or G3BP1 knock-out (-/-), using G3BP1 (E9G1M) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 2: G3BP1 (E9G1M) XP<sup>®</sup> Rabbit mAb
Immunoprecipitation of G3BP1 protein from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is G3BP1 (E9G1M) XP® Rabbit mAb. Western blot analysis was performed using G3BP1 (E9G1M) XP® Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody.
Immunoprecipitation Image 1: G3BP1 (E9G1M) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

#61559

G3BP1 (E9G1M) XP® Rabbit mAb

CST Logo
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using G3BP1 (E9G1M) XP® Rabbit mAb.
Immunohistochemistry Image 1: G3BP1 (E9G1M) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using G3BP1 (E9G1M) XP® Rabbit mAb.
Immunohistochemistry Image 2: G3BP1 (E9G1M) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using G3BP1 (E9G1M) XP® Rabbit mAb.
Immunohistochemistry Image 3: G3BP1 (E9G1M) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

#61559

G3BP1 (E9G1M) XP® Rabbit mAb

CST Logo
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using G3BP1 (E9G1M) XP® Rabbit mAb.
Immunohistochemistry Image 4: G3BP1 (E9G1M) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using G3BP1 (E9G1M) XP® Rabbit mAb.
Immunohistochemistry Image 5: G3BP1 (E9G1M) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using G3BP1 (E9G1M) XP® Rabbit mAb.
Immunohistochemistry Image 6: G3BP1 (E9G1M) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

#61559

G3BP1 (E9G1M) XP® Rabbit mAb

CST Logo
Immunohistochemical analysis of paraffin-embedded human ovarian clear cell carcinoma using G3BP1 (E9G1M) XP® Rabbit mAb.
Immunohistochemistry Image 7: G3BP1 (E9G1M) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded normal human thymus using G3BP1 (E9J1M) XP® Rabbit mAb.
Immunohistochemistry Image 8: G3BP1 (E9G1M) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded normal human testis using G3BP1 (E9J1M) XP® Rabbit mAb.
Immunohistochemistry Image 9: G3BP1 (E9G1M) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

#61559

G3BP1 (E9G1M) XP® Rabbit mAb

CST Logo
Immunohistochemical analysis of paraffin-embedded normal human lymph node using G3BP1 (E9J1M) XP® Rabbit mAb.
Immunohistochemistry Image 10: G3BP1 (E9G1M) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human appendix using G3BP1 (E9G1M) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 11: G3BP1 (E9G1M) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, untreated (left) or treated with sodium arsenite (right), using G3BP1 (E9G1M) XP® Rabbit mAb.
Immunohistochemistry Image 12: G3BP1 (E9G1M) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

#61559

G3BP1 (E9G1M) XP® Rabbit mAb

CST Logo
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with sodium arsenite (500 µM, 30 min; right), using G3BP1 (E9G1M) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 1: G3BP1 (E9G1M) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.