DLX1 (E4T1L) Rabbit mAb

Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

[email protected]

cellsignal.com

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IF-F, IF-IC

Reactivity:
H M R

Sensitivity:
Endogenous

MW (kDa):
28

Source/Isotype:
Rabbit IgG

UniProt ID:
#P56177

Entrez-Gene Id:
1745

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunofluorescence (Frozen)	1:200 - 1:800
Immunofluorescence (Immunocytochemistry)	1:400 - 1:1600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

DLX1 (E4T1L) Rabbit mAb recognizes endogenous levels of total DLX1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His245 of human DLX1 protein.

Background

Homeobox protein DLX-1 (DLX1) is a member of a family of homeobox genes related to the Drosophila distal-less gene. DLX1 is a transcription factor found in the forebrain of the developing mouse embryo, where it contributes to craniofacial development (1-3). DLX1 also plays a critical role in GABAergic neurogenesis and retinal development (4-7). The DNA-binding activity of DLX1 is mediated by its homeodomain, and DLX1 has been shown to bind to genes that promote neuronal migration and repress neurite growth (8,9). DLX1 expression is regulated by various non-coding RNAs in different cancer types (10-12).

Background References

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

W: Western Blotting IF-F: Immunofluorescence (Frozen)

Cross-Reactivity Key

H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明，且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的，或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的，或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com

For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

#96585

DLX1 (E4T1L) Rabbit mAb

Western blot analysis of extracts from various cell lines using DLX1 (E4T1L) Rabbit mAb (upper) or GAPDH (D16H11) XP^® Rabbit mAb #5174 (lower).

Western Blotting Image 1: DLX1 (E4T1L) Rabbit mAb

Confocal immunofluorescent analysis of fixed frozen E12.5 mouse embryo, labeled with DLX1 (E4T1L) Rabbit mAb (left, green) and co-labeled with DyLight 554 Phalloidin #13054 (right, red) and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).

Immunofluorescence Image 1: DLX1 (E4T1L) Rabbit mAb

Confocal immunofluorescent analysis of A2780 cells (left, positive) and IMR-32 cells (right, negative) using DLX1 (E4T1L) Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red), and DAPI #4083 (blue).

Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com

For Research Use Only. Not for Use in Diagnostic Procedures.