Revision 1

#47074

Store at -20C

CD162/PSGL-1 (F8D5X) Rabbit mAb
CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP, FC-L

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
240, 120

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q14242

Entrez-Gene Id:
6404

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Flow Cytometry (Live) 1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

CD162/PSGL-1 (F8D5X) Rabbit mAb recognizes endogenous levels of total CD162/PSGL-1 protein. This antibody reacts with monomeric and dimeric forms of CD162/PSGL-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly281 of human CD162/PSGL-1 protein.

Background

P-selectin glycoprotein ligand-1 (PSGL-1/CD162) is a disulfide-linked homodimer expressed on the cell surface of lymphoid and myeloid cells as a type I transmembrane sialoglycoprotein (1,2). The mature protein backbone of the extracellular domain of PSGL-1 is extensively modified with N-linked glycans, O-linked glycans, sulfated tyrosine residues, and sialyl-Lewis X epitopes (3). Collectively, these modifications modulate high affinity interactions between PSGL-1 and multiple selectins, which facilitates immune cell trafficking to primary and secondary lymphoid organs (4-8).

Background References

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation FC-L: Flow Cytometry (Live)

Cross-Reactivity Key

H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品 ， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

#47074

CD162/PSGL-1 (F8D5X) Rabbit mAb

CST Logo
Western blot analysis of extracts from various human cell lines using CD162/PSGL-1 (F8D5X) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Negative expression of CD162/PSGL-1 protein in NCI-H209 cells is consistent with the predicted expression pattern. KARPAS 299 cell line source: Dr. Abraham Karpas at the University of Cambridge.
Western Blotting Image 1: CD162/PSGL-1 (F8D5X) Rabbit mAb
Immunoprecipitation of CD162/PSGL-1 protein from KARPAS 299 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CD162/PSGL-1 (F8D5X) Rabbit mAb. Western blot analysis was performed using CD162/PSGL-1 (F8D5X) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. KARPAS 299 cell line source: Dr. Abraham Karpas at the University of Cambridge.
Immunoprecipitation Image 1: CD162/PSGL-1 (F8D5X) Rabbit mAb
Flow cytometric analysis of live human peripheral blood mononuclear cells using CD162/PSGL-1 (F8D5X) Rabbit mAb (right) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left), co-stained with CD3 (UCHT1) Mouse mAb (APC Conjugate) #19881. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Flow Cytometry Image 1: CD162/PSGL-1 (F8D5X) Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

#47074

CD162/PSGL-1 (F8D5X) Rabbit mAb

CST Logo
Flow cytometric analysis of live 293T cells (blue, negative) and KARPAS 299 cells (green, positive) using CD162/PSGL-1 (F8D5X) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Flow Cytometry Image 2: CD162/PSGL-1 (F8D5X) Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.