Revision 1

#98783

Store at -20C

BAF (E4O8R) Rabbit mAb
CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IF-IC

Reactivity:
H M R Mk

Sensitivity:
Endogenous

MW (kDa):
10,11

Source/Isotype:
Rabbit IgG

UniProt ID:
#O75531

Entrez-Gene Id:
8815

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Immunocytochemistry) 1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

BAF (E4O8R) Rabbit mAb recognizes endogenous levels of total BAF protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala12 of human BAF protein.

Background

Barrier-to-autointegration factor (BAF or BANF1) plays a major role in the lifecycle of retroviruses, where it inhibits the reverse transcribed viral genome from integrating into itself (1-3). BAF exists as a dimer in solution, and serves as a bridge for double-stranded DNA in an ordered nucleoprotein complex (4). BAF interacts with LEM-domains of lamin binding proteins emerin and LAP2, and serves to help reform the nuclear envelope during telophase (5-7). The binding of BAF to its various partners is inhibited by Ser4 phosphorylation, which is deposited by vaccinia-related kinases (VRKs) and occurs during interphase and mitosis (8,9). Certain viral genomes encode kinases extremely similar to VRK to prevent BAF from binding to their DNA, impeding viral replication (10,11). BAF has been implicated in various tumor types and is associated with poor prognosis (12-14).

Background References

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.