Revision 1

#99209

Store at -20C

Axl (E3Z2H) Rabbit mAb (BSA and Azide Free)
CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IHC-P

Reactivity:
H M R

Sensitivity:
Endogenous

MW (kDa):
138 kDa (human), 133 kDa (mouse)

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q00993

Entrez-Gene Id:
26362

Product Usage Information

This product is the carrier free version of product #18554. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #18554

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity/Sensitivity

Axl (E3Z2H) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total Axl protein by western blot. This antibody is not suggested for immunohistochemical analysis of human tissues.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse Axl protein.

Background

Axl, Sky, and Mer are three members of a receptor tyrosine kinase (RTK) family that share a conserved intracellular tyrosine kinase domain and an extracellular domain similar to those seen in cell adhesion molecules. These RTKs bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6), which is structurally related to the protein S anticoagulation factor (1). Upon binding to its receptor, Gas6 activates phosphatidylinositol 3-kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-κB (2,3). A large body of evidence supports a role for Gas6/Axl signaling in cell growth and survival in normal and cancer cells (4).

Background References

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

W: Western Blotting IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: Human M: Mouse R: Rat

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品 ， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

#99209

Axl (E3Z2H) Rabbit mAb (BSA and Azide Free)

CST Logo
Western blot analysis of extracts from various cell lines using Axl (E3Z2H) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of Axl protein in Neuro-2a and SK-MEL-28 cells is consistent with the predicted expression pattern. Data were generated using the standard formulation of this product.
Western Blotting Image 1: Axl (E3Z2H) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using Axl (E3Z2H) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: Axl (E3Z2H) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded GL261 syngeneic tumor using Axl (E3Z2H) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: Axl (E3Z2H) Rabbit mAb (BSA and Azide Free)
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

#99209

Axl (E3Z2H) Rabbit mAb (BSA and Azide Free)

CST Logo
Immunohistochemical analysis of paraffin-embedded CT26 syngeneic tumor using Axl (E3Z2H) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: Axl (E3Z2H) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse liver using Axl (E3Z2H) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: Axl (E3Z2H) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse spleen using Axl (E3Z2H) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: Axl (E3Z2H) Rabbit mAb (BSA and Azide Free)
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

#99209

Axl (E3Z2H) Rabbit mAb (BSA and Azide Free)

CST Logo
Immunohistochemical analysis of paraffin-embedded human mouse spleen (left), lung (middle), or MC38 syngeneic tumor (right) using Axl (E3Z2H) Rabbit mAb (top) or an Axl Rabbit mAb (bottom). These two antibodies detect unique, non-overlapping epitopes on mouse Axl. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: Axl (E3Z2H) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mammary tumor using Axl (E3Z2H) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).  Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: Axl (E3Z2H) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded NIH/3T3 cell pellet (left, positive) or Neuro-2a cell pellet (right, negative) using Axl (E3Z2H) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 8: Axl (E3Z2H) Rabbit mAb (BSA and Azide Free)
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.