Revision 6

#92479

Store at -20C

CST Logo
Orders:

877-616-CELL (2355)

orders@cellsignal.com

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP, IHC-Bond, IHC-P, FC-FP

Reactivity:
H M R

Sensitivity:
Endogenous

MW (kDa):
64

Source/Isotype:
Rabbit IgG

UniProt ID:
#P08243

Entrez-Gene Id:
440

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
IHC Leica Bond 1:250 - 1:1000
Immunohistochemistry (Paraffin) 1:250 - 1:1000
Flow Cytometry (Fixed/Permeabilized) 1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #81128.

Specificity/Sensitivity

Asparagine Synthetase (E6C2C) XP® Rabbit mAb recognizes endogenous levels of total asparagine synthetase protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys467 of human asparagine synthetase protein.

Background

Asparagine synthetase (ASNS) catalyzes the synthesis of asparagine from aspartate and glutamine. Research studies have shown that intracellular asparagine can suppress apoptosis in a large number of human tumors. In addition, ASNS expression levels have been associated with the progression of gliomas and neuroblastomas in patients (1). Furthermore, acute lymphocytic leukemia cells frequently depend upon serum asparagine for their viability, as they lack ASNS (2). Deprivation of asparagine by L-asparaginase has therefore been developed as a therapeutic treatment for acute lymphocytic leukemia (2,3). In subsets of gastric and hepatic cancers, ASNS promoter hypermethylation correlates with low ASNS expression, sensitizing these cancers to the asparaginase treatment (4).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin) FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: Human M: Mouse R: Rat

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意,否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件,除非书 CST 的合法授书代表以书面形式书独接受, 否书均被拒书,并且无效。

专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品 , (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 6

Cell Signaling Technology Logo
Western blot analysis of extracts from K-562 and Loucy cells using Asparagine Synthetase (E6C2C) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The absence of detectable asparagine synthetase in Loucy cells is consistent with published data (5), confirming the specificity of the antibody for asparagine synthetase.
Western Blotting Image 1: Asparagine Synthetase (E6C2C) XP<sup>®</sup> Rabbit mAb
Western blot analysis of extracts from HCT 116, TALL-1, and JHH-5 cells using Asparagine Synthetase (E6C2C) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The absence of detectable asparagine synthetase in TALL-1 and JHH-5 cells is consistent with published data (5) and publicly available RNAseq data, respectively, confirming the specificity of the antibody for asparagine synthetase.
Western Blotting Image 2: Asparagine Synthetase (E6C2C) XP<sup>®</sup> Rabbit mAb
Immunoprecipitation of asparagine synthetase protein from OVCAR-4 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Asparagine Synthetase (E6C2C) XP® Rabbit mAb. Western blot analysis was performed using Asparagine Synthetase (E6C2C) XP® Rabbit mAb. Mouse Anti-Rabbit IgG (Light-Chain Specific) (D4W3E) mAb (HRP Conjugate) #93702 was used as the secondary antibody.
Immunoprecipitation Image 1: Asparagine Synthetase (E6C2C) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 6

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human oropharyngeal squamous cell carcinoma using Asparagine Synthetase (E6C2C) XP® Rabbit mAb performed on the Leica BOND RX.

Immunohistochemistry Image 1: Asparagine Synthetase (E6C2C) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Asparagine Synthetase (E6C2C) XP® Rabbit mAb performed on the Leica BOND RX.

Immunohistochemistry Image 2: Asparagine Synthetase (E6C2C) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using Asparagine Synthetase (E6C2C) XP® Rabbit mAb performed on the Leica BOND RX.
Immunohistochemistry Image 3: Asparagine Synthetase (E6C2C) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 6

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Asparagine Synthetase (E6C2C) XP® Rabbit mAb.
Immunohistochemistry Image 1: Asparagine Synthetase (E6C2C) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin lymphoma using Asparagine Synthetase (E6C2C) XP® Rabbit mAb.
Immunohistochemistry Image 2: Asparagine Synthetase (E6C2C) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Asparagine Synthetase (E6C2C) XP® Rabbit mAb.
Immunohistochemistry Image 3: Asparagine Synthetase (E6C2C) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 6

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded JHH-5 xenograft using Asparagine Synthetase (E6C2C) XP® Rabbit mAb. Note the presence of signal in the mouse immune cells and lack of signal in the ASNS negative JHH-5 cells.
Immunohistochemistry Image 4: Asparagine Synthetase (E6C2C) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded mouse pancreas using Asparagine Synthetase (E6C2C) XP® Rabbit mAb.
Immunohistochemistry Image 5: Asparagine Synthetase (E6C2C) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded mouse spleen using Asparagine Synthetase (E6C2C) XP® Rabbit mAb.
Immunohistochemistry Image 6: Asparagine Synthetase (E6C2C) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 6

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Asparagine Synthetase (E6C2C) XP® Rabbit mAb (left) or Asparagine Synthetase Rabbit mAb (right). These two antibodies detect independent, unique epitopes on human asparagine synthetase. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Immunohistochemistry Image 7: Asparagine Synthetase (E6C2C) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human pancreas using Asparagine Synthetase (E6C2C) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 8: Asparagine Synthetase (E6C2C) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded HCT 116 cell pellet (left, positive) or TALL-1 cell pellet (right, negative) using Asparagine Synthetase (E6C2C) XP® Rabbit mAb.
Immunohistochemistry Image 9: Asparagine Synthetase (E6C2C) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 6

Cell Signaling Technology Logo
Flow cytometric analysis of Loucy cells (blue) and K-562 cells (green) using Asparagine Synthetase (E6C2C) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Flow Cytometry Image 1: Asparagine Synthetase (E6C2C) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.