Revision 1
#74806
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IF-F
Reactivity:
H M R
Sensitivity:
Endogenous
MW (kDa):
28
Source/Isotype:
Mouse IgG2b kappa
UniProt ID:
#P55087
Entrez-Gene Id:
361
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunofluorescence (Frozen) | 1:200 - 1:800 |
Storage
Specificity/Sensitivity
Source / Purification
Background
AQP4 is expressed in brain cells, including neurons, but enriched in astrocytes, as well as in the peripheral nervous system (4,7). AQP4 influences synaptic plasticity, and the lack of this protein in the brain may cause memory and learning impairment via glutamate transporter 1 (GLT1) in Alzheimer's disease (8-10). It's suggested that AQP4 could play a role in the clearance of β-amyloid, and it may influence the transport of potassium and calcium in Alzheimer's disease (8,9). In Parkinson's disease, the lack of AQP4 results in an increased susceptibility of neurons from the substantia nigra and the ventral tegmental area for MPTP (11).
Background References
- Takata, K. et al. (2004) Prog Histochem Cytochem 39, 1-83.
- Echevarría, M. and Ilundáin, A.A. (1998) J Physiol Biochem 54, 107-18.
- Gomes, A. et al. (2018) Front Chem 6, 238.
- Xu, M. et al. (2017) Adv Exp Med Biol 969, 81-103.
- Kobayashi, H. et al. (2004) J Pharmacol Sci 96, 264-70.
- Meli, R. et al. (2018) Front Physiol 9, 101.
- Ishibashi, K. et al. (1998) Biochem Biophys Res Commun 244, 268-74.
- Hubbard, J.A. et al. (2018) Brain Res Bull 136, 118-129.
- Lan, Y.L. et al. (2016) Neural Plast 2016, 4626593.
- Lan, Y.L. et al. (2016) Mol Neurobiol 53, 5300-9.
- Zhang, J. et al. (2016) Neurosci Lett 614, 7-15.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IF-F: Immunofluorescence (Frozen)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
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