Revision 1
#14175
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W
Reactivity:
H Mk
Sensitivity:
Endogenous
MW (kDa):
110, 150
Source/Isotype:
Rabbit IgG
UniProt ID:
#P55265
Entrez-Gene Id:
103
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity/Sensitivity
Species predicted to react based on 100% sequence homology
Source / Purification
Background
ADAR1 is ubiquitously expressed with two known isoforms, ADAR1L (p150) and ADAR1S (p110), resulting from transcription using alternative promoters and start codons. ADAR1S is constitutively expressed in the nucleus, while ADAR1L is interferon-inducible and present in both the nucleus and the cytoplasm. The induction of ADAR1L in response to cellular stress and viral infection suggests a role for RNA editing in the innate immune response (1,7). In addition, ADAR1 is essential in mammalian development, particularly in hematopoiesis and suppression of interferon signaling to protect hematopoietic stem cells from destruction in fetal liver and adult bone marrow (8,9).
Background References
- Zinshteyn, B. and Nishikura, K. (2009) Wiley Interdiscip Rev Syst Biol Med 1, 202-9.
- Nishikura, K. (2006) Nat Rev Mol Cell Biol 7, 919-31.
- Bass, B.L. (2002) Annu Rev Biochem 71, 817-46.
- Reenan, R.A. (2001) Trends Genet 17, 53-6.
- Maas, S. et al. (2006) RNA Biol 3, 1-9.
- Rueter, S.M. et al. (1999) Nature 399, 75-80.
- Patterson, J.B. and Samuel, C.E. (1995) Mol Cell Biol 15, 5376-88.
- Iizasa, H. and Nishikura, K. (2009) Nat Immunol 10, 16-8.
- Hartner, J.C. et al. (2009) Nat Immunol 10, 109-15.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
CST is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
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