Revision 4
#14194
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877-616-CELL (2355)
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3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
68, 90
Source/Isotype:
Rabbit
UniProt ID:
#O14672
Entrez-Gene Id:
102
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity/Sensitivity
Source / Purification
Background
The ADAM metallopeptidase domain 10 (ADAM10) is a plasma membrane proteinase that cleaves membrane-bound proteins targeted for regulated intramembrane proteolysis (RIP). The ADAM10 prodomain acts as a chaperone that stabilizes mature ADAM protein folding, and prevents target-protein shedding through inhibition of ADAM10 proteinase activity (3,4). Mature ADAM10 is the major α-secretase responsible for cleavage of Notch, APP, cadherins, and prion protein (5-7). The ADAM10 protein cleaves receptor tyrosine kinases and their associated ligands and displays a wide range of regulatory functions across related signaling pathways (8). Research studies using knockout mice demonstrate that loss of ADAM10 results in defects in cortex formation, lymphocyte development, and cardiovascular development (9-11). Increased ADAM10 protein expression correlates with progression of many types of cancer (i.e. gastric cancer, hepatocellular carcinoma, and brain glioma), due to increased cancer cell migration, metastasis, and invasion (12-14). Mutations in the corresponding ADAM10 gene result in a rare, autosomal dominant pigmentation disorder known as reticulate acropigmentation of Kitamura (15).
Background References
- Turner, A.J. and Hooper, N.M. (1999) Biochem Soc Trans 27, 255-9.
- Edwards, D.R. et al. (2008) Mol Aspects Med 29, 258-89.
- Suh, J. et al. (2013) Neuron 80, 385-401.
- Moss, M.L. et al. (2007) J Biol Chem 282, 35712-21.
- Hartmann, D. et al. (2002) Hum Mol Genet 11, 2615-24.
- Miller, M.A. et al. (2013) Proc Natl Acad Sci U S A 110, E2074-83.
- Reiss, K. et al. (2005) EMBO J 24, 742-52.
- Endres, K. et al. (2009) Neurobiol Dis 36, 233-41.
- Jorissen, E. et al. (2010) J Neurosci 30, 4833-44.
- Zhang, C. et al. (2010) Dev Dyn 239, 2594-602.
- Gibb, D.R. et al. (2011) Mol Immunol 48, 1319-27.
- Wang, Y.Y. et al. (2011) J Surg Oncol 103, 116-23.
- Yuan, S. et al. (2013) Oncol Rep 30, 1715-22.
- Bulstrode, H. et al. (2012) Cancer Lett 326, 79-87.
- Kono, M. et al. (2013) Hum Mol Genet 22, 3524-33.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
限制使用
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Revision 4
#14194