FastScan^™ Total Neurogranin ELISA Kit

The FastScan™ Total Neurogranin ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of neurogranin. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with neurogranin in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of neurogranin.

*Antibodies in kit are custom formulations specific to kit.

The FastScan™ Total Neurogranin ELISA Kit detects endogenous levels of neurogranin, as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Neurogranin is a small neuronal protein that binds to calmodulin in the absence of Ca²⁺ (1,2). Calmodulin binds Ca²⁺ and acts as a Ca²⁺ sensor to drive Ca²⁺-dependent signal transduction pathways by modulating its interaction with various kinases and phosphatases (3). In the postsynaptic compartment of the neuron, Ca²⁺ is carefully regulated and is a critical regulator of synaptic function and plasticity (4). Neurogranin is primarily expressed in the brain and is enriched in somato-dendritic compartments of projection neurons in various regions of the brain (5). Postsynaptically-enriched neurogranin likely influences Ca²⁺ or Ca²⁺/calmodulin-dependent neuronal functions, including synaptic plasticity, by binding to and releasing calmodulin in a Ca²⁺-dependent manner (6-9). Genetic variants in the gene encoding neurogranin, NRGN, are linked to several neuropsychiatric diseases, including schizophrenia (10). Interestingly, increase in cerebrospinal fluid neurogranin is correlated with neurodegenerative disease progression, which suggests that neurogranin could act as a biomarker for diseases like Alzheimer’s disease (11).