The rapid protocol (RP) PathScan^® RP ApoE2 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of ApoE2 in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with ApoE2 in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of ApoE2. Learn more about all of your ELISA kit options here.

*Antibodies in this kit are custom formulations specific to kit.

The PathScan^® RP ApoE2 Sandwich ELISA Kit detects endogenous levels of ApoE2 protein, but does not cross-react with ApoE3 or ApoE4, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Apolipoproteins are plasma lipoproteins that function as transporters of lipids and cholesterol in the circulatory system. Chylomicrons are a fundamental class of apolipoproteins containing very low-density lipoproteins (VLDL), intermediate-density lipoproteins (IDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) (1,2).
Human ApoE has three isoforms: ApoE2, ApoE3, and ApoE4. These three isoforms differ in the combination of cysteine and arginine residues located at positions 130 and 176. The ApoE2 isoform contains cysteine at both locations. ApoE4 has been identified as a genetic risk factor for Alzheimer’s disease (AD), where ApoE2 is neuroprotective, reducing risk of AD by nearly 50%. The neuroprotective mechanism of ApoE2 remains under investigation, but studies suggest that this may be due to its overall protein abundance in non-AD human cortex and its unique lipid binding profile compared to ApoE3 and ApoE4 which may increase overall beta-amyloid clearance (3).