PathScan^® Phospho-SMAD2 (Ser465/467) Sandwich ELISA Kit

1 Kit

(96 assays)

Species Cross Reactivity:
H M Mi

UniProt ID:
#Q15796

Entrez-Gene Id:
#4087

Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

[email protected]

cellsignal.com

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes	Product #	Quantity	Color	Storage Temp
SMAD2 Mouse mAb Coated Microwells	58908	96 tests		+4C
Phospho-SMAD2 (Ser465/467) Rabbit Detection mAb	13767	1 ea	Green (Lyophilized)	+4C
Anti-rabbit IgG, HRP-linked Antibody (ELISA Formulated)	13272	1 ea	Red (Lyophilized)	+4C
Detection Antibody Diluent	13339	11 ml	Green	+4C
HRP Diluent	13515	11 ml	Red	+4C
TMB Substrate	7004	11 ml		+4C
STOP Solution	7002	11 ml		+4C
Sealing Tape	54503	2 ea		+4C
ELISA Wash Buffer (20X)	9801	25 ml		+4C
ELISA Sample Diluent	11083	25 ml	Blue	+4C
Cell Lysis Buffer (10X)	9803	15 ml		-20C

*The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Description

The PathScan^® Phospho-SMAD2 (Ser465/467) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of SMAD2 when phosphorylated at Ser465/467. A SMAD2 Mouse monoclonal Antibody has been coated onto the microwells. After incubation with cell lysates, SMAD2 (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a Phospho-SMAD2 (Ser465/467) Detection Antibody is added to detect serine phosphorylation of the captured SMAD2 protein. Anti-rabbit IgG, HRP-Linked Ab is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of SMAD2 phosphorylated at Ser465/467.

*Antibodies in this kit are custom formulations specific to kit.

Specificity/Sensitivity

CST's PathScan^® Phospho-SMAD2 (Ser465/467) Sandwich ELISA Kit #7348 detects SMAD2 when phosphorylated at Ser465/467. As shown in Figure 1, a significant induction of SMAD2 phosphorylation at Ser465/467 can be detected in TGF-β-treated HaCaT cells using the PathScan^® Phospho-SMAD2 (Ser465/467) Sandwich ELISA Kit #7348. The level of total SMAD2 (phospho and nonphospho) remains unchanged as shown by western analysis and by PathScan^® Total SMAD2 Sandwich ELISA Kit #7244 (Figure 1). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Background

Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include SMAD1, 2, 3, 5, and 9; the common-mediator SMAD (co-SMAD), SMAD4; and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7 (1-5). Activated type I receptors associate with specific R-SMADs and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-SMADs dissociate from the receptor and form a heteromeric complex with SMAD4, initiating translocation of the heteromeric SMAD complex to the nucleus. Once in the nucleus, SMADs recruit a variety of DNA binding proteins that function to regulate transcriptional activity (6-8).

Background References

Applications Key

ELISA: ELISA

Cross-Reactivity Key

H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PathScan is a registered trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com