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Rat Vascular Endothelial Growth Factor-164 (rVEGF₁₆₄ )

Rat Vascular Endothelial Growth Factor-164 (rVEGF₁₆₄ ) #5874

Name: Rat Vascular Endothelial Growth Factor-164 (rVEGF 164 )
Price: 238.00 USD
Availability: InStock

Product Information

Product Usage Information

Reconstitution:

With carrier: Add sterile PBS or PBS containing 1% bovine or human serum albumin or 5-10% FBS to a final rVEGF164 concentration of greater than 50 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing.

Carrier free: Add sterile PBS or PBS containing protein to minimize absorption of rVEGF164 to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock rVEGF164 should be greater than 50 μg/ml.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg rVEGF₁₆₄. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Storage

Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

Purity	>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant rVEGF₁₆₄. All lots are greater than 98% pure.
Endotoxin	Less than 0.01 ng endotoxin/1 μg rVEGF₁₆₄.
Activity	The bioactivity of recombinant rVEGF₁₆₄ was determined in a cell proliferation assay using HUVEC. The ED₅₀ of each lot is between 1-5 ng/ml.
Molecular Formula	Recombinant rVEGF₁₆₄ contains no "tags" and the nonglycosylated protein has a calculated MW of 19,234. DTT-reduced protein migrates as a 24-31 kDa polypeptide. Lower mobility in SDS-PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 46-53 kDa protein. The expected amino-terminal APTTE of recombinant rVEGF₁₆₄ was verified by amino acid sequencing.

Source / Purification

Recombinant rat VEGF₁₆₄ (rVEGF₁₆₄) Ala27-Arg190 (Accession #NP_001103803) was expressed in human 293 cells at Cell Signaling Technology.

Background

VEGF₁₆₄ is one of many splice variants of the VEGF-A gene, and is one amino acid shorter than its human counterpart, VEGF₁₆₅ (1, 2). VEGF₁₆₄ is produced by a number of cells including endothelial cells, macrophages, and T cells (1, 2). VEGF₁₆₄ is involved in angiogenesis, vascular endothelial cell survival, growth, migration, and vascular permeability (1,2). Gene expression is induced by hypoxia, inflammatory cytokines, and oncogenes (1, 2). VEGF₁₆₄ binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix (1-3). VEGFR1 and VEGFR2 are the receptor tyrosine kinases for VEGF₁₆₄ (2). NRP-1 and NRP-2 may function as co-receptors and enhance VEGFR2 signaling (2-3). Binding of VEGF₁₆₄ to VEGFR1 and VEGFR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK, and Paxillin (2).

Database Links

UniProt ID: P16612

Entrez-Gene Id: 83785

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Rat Vascular Endothelial Growth Factor-164 (rVEGF164 ) #5874