Revision 1

#74319

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

MW (kDa):
15.1

UniProt ID:
#P05112

Entrez-Gene Id:
3565

Background

Interleukin-4 (IL-4) is a cytokine secreted by activated T cells, basophils, and mast cells (1,2). While it contributes to many immunomodulatory responses, it is mainly recognized as the cytokine responsible for eliciting differentiation of naive T cells into Th2 lineage cells that are defined by their secretion of IL-4, IL-5, and IL-10 (3). In addition, IL-4 contributes to immunoglobulin class switching by inducing the production of IgE from B cells (4,5). IL-4 acts through the IL-4 receptor, leading to tyrosine phosphorylation and activation of the Stat6 transcription factor (6).

Endotoxin

Endotoxin levels are less than or equal to 1 EU / 1 μg hIL-4.

Purity

A greater than or equal to 95% purity was determined by SDS-PAGE.

Source / Purification

Recombinant human IL-4 was expressed in E. coli and is supplied in a lyophilized form.

Bioactivity

The bioactivity of recombinant hIL-4 was determined in a TF-1 cell proliferation assay. The ED50 of each lot is less than or equal to 0.25 ng/mL.

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Serial dilutions of Human IL-4 Recombinant Protein were added to TF-1 cells. Cell proliferation was measured and the linear portion of the curve was used to calculate the ED50.
undefined Image 1: Human IL-4 Recombinant Protein
The purity of Human IL-4 Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant hIL-4 and staining with Coomassie Blue.
undefined Image 2: Human IL-4 Recombinant Protein
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.