V5-Tag (E9H8O) Mouse mAb (Magnetic Bead Conjugate) #53475
Filter:
- IP
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | |
| Source/Isotype | Mouse IgG2a kappa |
Application Key:
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Description
This Cell Signaling Technology antibody is immobilized by the covalent reaction of formylbenzamide-modified antibody with hydrazide-activated magnetic bead. V5-Tag (E9H8O) Mouse mAb (Magnetic Bead Conjugate) is useful for immunoprecipitation assays of V5-tagged recombinant proteins. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated V5-Tag (E9H8O) Mouse mAb #80076.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunoprecipitation | 1:20 |
Storage
Supplied in PBS Buffer (pH 7.2), 0.1% Tween® 20. Store at 4°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
V5-Tag (E9H8O) Mouse mAb recognizes transfected levels of recombinant protein containing the V5 epitope tag. This antibody also recognizes a non-specific band of unknown origin at 21 kDa.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic V5-tag peptide.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
The V5-tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family (1). The V5-tag can be used to detect expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.
The V5-tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family (1). The V5-tag can be used to detect expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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